ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (5): 1041-1048.doi: 10.11843/j.issn.0366-6964.2016.05.023

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Molecular Cloning,Expression Pattern and Function of Duck I-FABP Gene

CHEN Fang1,2,3* ,ZHANG Hao1,2,3,LU Li-zhi3   

  1. (1.Institute of Animal Husbandry and Veterinary Sciences,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;2.Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Wuhan 430064,China;3.Institute of Animal Husbandry and Veterinary Sciences,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
  • Received:2015-06-25 Online:2016-05-23 Published:2016-05-23

Abstract:

This study aimed to analyze the structure and function of duck I-FABP gene.The Shaoxing duck was used.The sequence of duck I-FABP mRNA was cloned by RACE and RT-PCR.The expression patterns of I-FABP in different parts of small intestine were detected by qRT-PCR.The function was analyzed by siRNA and qRT-PCR.The duck I-FABP gene encodes a 132-amino acid protein.A mutation from T to C resulting in a substitution from Ile to Thr was found at codon 60 in exon 2 of the I-FABP.I-FABPMTTP and ApoB mRNA had high expression levels in duodenum and the first half of jejunum and had slight differences with the expression of L-FABP.After the I-FABP interfered,the expression of MTTP and ApoB were decreased significantly,but no obvious change at the expression level of L-FABP.The results verify the important role of I-FABP in uptake and translocation of fatty acid in small intestine of ducks and provide the foundation for further research.

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